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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli

Fig. 5

Comparison of acetoin production with the expression of ilvBN and aldB under control of the 3 synthetic nar promoters in flask cultures. a Combination of expression modules of ilvBN and aldB genes. Modules are Si, Ii, Wi, Sa, Ia, and Wa; S stands for strong promoter, I for intermediate promoter, W for weak promoter, i for ilvBN, and a for aldB. b Titer of acetoin obtained by expressing 10 modules in flask cultures. The positive control Ni–Na is a module expressing the ilvBN and aldB genes with a wild-type nar promoter. Black and white bars indicate acetoin and 2,3-BDO, respectively

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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