Biotechnology for Biofuels and Bioproducts

Table 3 Determination of the maximal adsorption of GFP₂ and probe proteins on several isolated lignins by Langmuir equation

From: Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein

Lignin	GFP₂ (no CBM)			His6–CBM–GFP₂
Lignin	A_max (μg/mg)	K _p	R ²	A_max (μg/mg)	K _p	R ²
SBML	18.1 ± 3.3	0.0028 ± 0.0010	0.9907	19.0 ± 4.3	0.00516 ± 0.00165	0.8605
PAL	18.9 ± 4.9	0.1286 ± 0.0044	0.9146	105.7 ± 23.9	0.0456 ± 0.0120	0.9373
WSKL	34.3 ± 8.0	0.0421 ± 0.0124	0.8853	170.9 ± 20.0	0.01432 ± 0.0022	0.9793

No BSA blocking was used in these experiments
SBML sugarcane bagasse milled lignin, PAL poplar alkaline lignin, WSKL wheat straw Klason lignin

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ISSN: 2731-3654

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