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Table 3 Determination of the maximal adsorption of GFP2 and probe proteins on several isolated lignins by Langmuir equation

From: Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein

Lignin GFP2 (no CBM) His6–CBM–GFP2
Amax (μg/mg) K p R 2 Amax (μg/mg) K p R 2
SBML 18.1 ± 3.3 0.0028 ± 0.0010 0.9907 19.0 ± 4.3 0.00516 ± 0.00165 0.8605
PAL 18.9 ± 4.9 0.1286 ± 0.0044 0.9146 105.7 ± 23.9 0.0456 ± 0.0120 0.9373
WSKL 34.3 ± 8.0 0.0421 ± 0.0124 0.8853 170.9 ± 20.0 0.01432 ± 0.0022 0.9793
  1. No BSA blocking was used in these experiments
  2. SBML sugarcane bagasse milled lignin, PAL poplar alkaline lignin, WSKL wheat straw Klason lignin