Table 1 S. cerevisiae strains and plasmids used in this study

Plasmids

 pUG6

The plasmid with LoxP–KanMX4–LoxP cassette

[38]

 pJX7

Yeast 2μ plasmid, TEF1p–Ru-xylA–PGK1t, URA3 marker

[19]

 YEp-CH

Shuttle plasmid for E. coli and S. cerevisiae, Cre gene under control of GAL2 promoter, Hygromycin marker

Laboratory preserved

 pIYC04

Yeast 2μ plasmid, PGK1p–CYC1t, TEF1p–ADHt, HIS3 marker

[19]

 pXIδ

Yeast 2μ plasmid, 3XI, δ1δ2, KanMX4 marker

[6]

 pUC20

Yeast 2μ plasmid, δ1δ2, TEF1p–ADHt, KanMX4 marker

This study

 pUC20–FBA1

pUC20, TEF1p–FBA1–ADHt

This study

 pUC20–TDH2

pUC20, TEF1p–TDH2–ADHt

This study

 pUC20–GPM1

pUC20 TEF1p–GPM1–ADHt

This study

 pUC20-RPL7A

pUC20 TEF1p-RPL7A -ADHt

This study

 pUC20–RPL7B

pUC20 TEF1p-RPL7B–ADHt

This study

 pUC20–RPL9A

pUC20 TEF1p–RPL9A–ADHt

This study

 pUC20–RPL22A

pUC20 TEF1p–RPL22A –ADHt

This study

 pUC20–RPL22B

pUC20 TEF1p–RPL22B–ADHt

This study

S. cerevisiae strains

 CEN.PK 113-5D

MATa; ura3-53

[36]

 XH7

Derived from a diploid S. cerevisiae strain isolated from tropical fruit in Thailand, pho13::XI, gre3::PPP, XK, 3δ::XI, AE^a

[6]

 BSGX001

CEN.PK 113-5D derivative; Ru-XI, XK, gre3::PPP, cox4Δ, AE^a

[39]

 BSGX001 (aca1Δ)^b

BSGX001, aca1::KanMX4

This study

 BSGX001 (RPL7A)^c

BSGX001, δ1-loxp–KanMX4–loxp–TEF1p-RPL7A–ADHt-δ2

This study