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Table 1 S. cerevisiae strains and plasmids used in this study

From: Disruption of the transcription factors Thi2p and Nrm1p alleviates the post-glucose effect on xylose utilization in Saccharomyces cerevisiae

S. cerevisiae strains and plasmids Description Sources
Plasmids
 pUG6 The plasmid with LoxPKanMX4LoxP cassette [38]
 pJX7 Yeast 2μ plasmid, TEF1pRu-xylAPGK1t, URA3 marker [19]
 YEp-CH Shuttle plasmid for E. coli and S. cerevisiae, Cre gene under control of GAL2 promoter, Hygromycin marker Laboratory preserved
 pIYC04 Yeast 2μ plasmid, PGK1pCYC1t, TEF1pADHt, HIS3 marker [19]
 pXIδ Yeast 2μ plasmid, 3XI, δ1δ2, KanMX4 marker [6]
 pUC20 Yeast 2μ plasmid, δ1δ2, TEF1pADHt, KanMX4 marker This study
 pUC20–FBA1 pUC20, TEF1pFBA1ADHt This study
 pUC20–TDH2 pUC20, TEF1pTDH2ADHt This study
 pUC20–GPM1 pUC20 TEF1pGPM1ADHt This study
 pUC20-RPL7A pUC20 TEF1p-RPL7A -ADHt This study
 pUC20–RPL7B pUC20 TEF1p-RPL7BADHt This study
 pUC20–RPL9A pUC20 TEF1pRPL9A–ADHt This study
 pUC20–RPL22A pUC20 TEF1pRPL22AADHt This study
 pUC20–RPL22B pUC20 TEF1pRPL22BADHt This study
S. cerevisiae strains
 CEN.PK 113-5D MATa; ura3-53 [36]
 XH7 Derived from a diploid S. cerevisiae strain isolated from tropical fruit in Thailand, pho13::XI, gre3::PPP, XK, 3δ::XI, AEa [6]
 BSGX001 CEN.PK 113-5D derivative; Ru-XI, XK, gre3::PPP, cox4Δ, AEa [39]
 BSGX001 (aca1Δ)b BSGX001, aca1::KanMX4 This study
 BSGX001 (RPL7A)c BSGX001, δ1-loxpKanMX4loxpTEF1p-RPL7AADHt-δ2 This study
  1. aAE adaptive evolution in medium using xylose as the sole carbon source
  2. bOther strains derived from BSGX001 with deleted genes were named in the same way, and because of lack of space they are not listed here
  3. cOther strains derived from BSGX001 with overexpressed genes were named in the same way, and because of lack of space they are not listed here