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Table 4 The fermentation characteristics of strains knocking out or overexpressing TFs whose expression levels were significantly different between the GX stage and X stage

From: Disruption of the transcription factors Thi2p and Nrm1p alleviates the post-glucose effect on xylose utilization in Saccharomyces cerevisiae

Genotype of strains

Glucose–xylose co-fermentation

Xylose fermentation

r axylose (g g−1 DCW h−1)

μ b

r cxylose (g g−1 DCW h−1)

r cethanol (g g−1 DCW h−1)

Control (BSGX001)

0.065 ± 0.002

0.082 ± 0.002

0.645 ± 0.001

0.216 ± 0.002

Positive operationd

 adr1Δ

0.046 ± 0.002*

0.075 ± 0.001*

0.642 ± 0.001*

0.235 ± 0.001*

 aca1Δ

0.054 ± 0.001*

0.083 ± 0.002*

0.667 ± 0.010

0.212 ± 0.001*

 znf1Δ

0.048 ± 0.001*

0.070 ± 0.001*

0.531 ± 0.008*

0.226 ± 0.002*

 nrg1Δ

0.023 ± 0.000*

0.067 ± 0.001*

0.488 ± 0.015

0.166 ± 0.003*

 rad16Δ

0.051 ± 0.001*

0.080 ± 0.002*

0.600 ± 0.002

0.244 ± 0.005*

 ypr196wΔ

0.039 ± 0.000*

0.080 ± 0.002*

0.650 ± 0.002*

0.257 ± 0.004*

 thi2Δ

0.109 ± 0.002*

0.069 ± 0.003*

0.505 ± 0.000*

0.206 ± 0.001*

 SWI5

0.038 ± 0.001*

0.078 ± 0.003*

0.610 ± 0.010

0.220 ± 0.001*

 SFG1

0.037 ± 0.002*

0.069 ± 0.000*

0.509 ± 0.001*

0.179 ± 0.002*

 YHP1

0.040 ± 0.001*

0.103 ± 0.002*

0.660 ± 0.001*

0.226 ± 0.001*

 NRM1

0.088 ± 0.001*

0.097 ± 0.001*

0.710 ± 0.002*

0.253 ± 0.001*

Negative operationd

 ADR1

0.013 ± 0.003*

0.082 ± 0.002*

0.664 ± 0.001*

0.173 ± 0.002*

 ACA1

0.051 ± 0.001*

0.080 ± 0.001*

0.600 ± 0.002*

0.200 ± 0.002*

 ZNF1

0.045 ± 0.001*

0.077 ± 0.002*

0.630 ± 0.002*

0.210 ± 0.001*

 NRG1

0.041 ± 0.000*

0.070 ± 0.003*

0.521 ± 0.002*

0.189 ± 0.003*

 RAD16

0.005 ± 0.001*

0.067 ± 0.002*

0.489 ± 0.001*

0.186 ± 0.005*

 YPR196W

0.031 ± 0.001*

0.080 ± 0.002*

0.650 ± 0.002*

0.257 ± 0.002*

 THI2

0.049 ± 0.000*

0.067 ± 0.000*

0.487 ± 0.002*

0.157 ± 0.000*

 swi5Δ

0.048 ± 0.002*

0.078 ± 0.002*

0.638 ± 0.004*

0.266 ± 0.001*

 sfg1Δ

0.053 ± 0.002*

0.066 ± 0.000*

0.487 ± 0.003*

0.169 ± 0.001*

 yhp1Δ

0.034 ± 0.002*

0.080 ± 0.001*

0.600 ± 0.002*

0.215 ± 0.001*

 nrm1Δ

0.015 ± 0.000*

0.080 ± 0.002*

0.637 ± 0.002*

0.266 ± 0.002*

  1. Cells were cultured at 30 °C in a shake flask and agitated at 200 rpm. All the data are the mean value ± standard deviation of independent triplicate tests
  2. * p < 0.05
  3. arxylose/rethanol was calculated from data on the xylose-only consumption phase in glucose–xylose co-fermentation (GX stage)
  4. bμ was calculated from data on the exponential growth phase of xylose fermentation (X stage)
  5. cThe specific consumption/production rates of xylose/ethanol (rxylose/rethanol) were calculated from data on X stage
  6. dThe positive operation represents overexpressed genes with lower expression in the GX stage compared to the X stage or knocked out genes with higher expression in the GX stage compared to the X stage; the negative operation represents the reverse operation
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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