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Table 4 The fermentation characteristics of strains knocking out or overexpressing TFs whose expression levels were significantly different between the GX stage and X stage

From: Disruption of the transcription factors Thi2p and Nrm1p alleviates the post-glucose effect on xylose utilization in Saccharomyces cerevisiae

Genotype of strains Glucose–xylose co-fermentation Xylose fermentation
r axylose (g g−1 DCW h−1) μ b r cxylose (g g−1 DCW h−1) r cethanol (g g−1 DCW h−1)
Control (BSGX001) 0.065 ± 0.002 0.082 ± 0.002 0.645 ± 0.001 0.216 ± 0.002
Positive operationd
 adr1Δ 0.046 ± 0.002* 0.075 ± 0.001* 0.642 ± 0.001* 0.235 ± 0.001*
 aca1Δ 0.054 ± 0.001* 0.083 ± 0.002* 0.667 ± 0.010 0.212 ± 0.001*
 znf1Δ 0.048 ± 0.001* 0.070 ± 0.001* 0.531 ± 0.008* 0.226 ± 0.002*
 nrg1Δ 0.023 ± 0.000* 0.067 ± 0.001* 0.488 ± 0.015 0.166 ± 0.003*
 rad16Δ 0.051 ± 0.001* 0.080 ± 0.002* 0.600 ± 0.002 0.244 ± 0.005*
 ypr196wΔ 0.039 ± 0.000* 0.080 ± 0.002* 0.650 ± 0.002* 0.257 ± 0.004*
 thi2Δ 0.109 ± 0.002* 0.069 ± 0.003* 0.505 ± 0.000* 0.206 ± 0.001*
 SWI5 0.038 ± 0.001* 0.078 ± 0.003* 0.610 ± 0.010 0.220 ± 0.001*
 SFG1 0.037 ± 0.002* 0.069 ± 0.000* 0.509 ± 0.001* 0.179 ± 0.002*
 YHP1 0.040 ± 0.001* 0.103 ± 0.002* 0.660 ± 0.001* 0.226 ± 0.001*
 NRM1 0.088 ± 0.001* 0.097 ± 0.001* 0.710 ± 0.002* 0.253 ± 0.001*
Negative operationd
 ADR1 0.013 ± 0.003* 0.082 ± 0.002* 0.664 ± 0.001* 0.173 ± 0.002*
 ACA1 0.051 ± 0.001* 0.080 ± 0.001* 0.600 ± 0.002* 0.200 ± 0.002*
 ZNF1 0.045 ± 0.001* 0.077 ± 0.002* 0.630 ± 0.002* 0.210 ± 0.001*
 NRG1 0.041 ± 0.000* 0.070 ± 0.003* 0.521 ± 0.002* 0.189 ± 0.003*
 RAD16 0.005 ± 0.001* 0.067 ± 0.002* 0.489 ± 0.001* 0.186 ± 0.005*
 YPR196W 0.031 ± 0.001* 0.080 ± 0.002* 0.650 ± 0.002* 0.257 ± 0.002*
 THI2 0.049 ± 0.000* 0.067 ± 0.000* 0.487 ± 0.002* 0.157 ± 0.000*
 swi5Δ 0.048 ± 0.002* 0.078 ± 0.002* 0.638 ± 0.004* 0.266 ± 0.001*
 sfg1Δ 0.053 ± 0.002* 0.066 ± 0.000* 0.487 ± 0.003* 0.169 ± 0.001*
 yhp1Δ 0.034 ± 0.002* 0.080 ± 0.001* 0.600 ± 0.002* 0.215 ± 0.001*
 nrm1Δ 0.015 ± 0.000* 0.080 ± 0.002* 0.637 ± 0.002* 0.266 ± 0.002*
  1. Cells were cultured at 30 °C in a shake flask and agitated at 200 rpm. All the data are the mean value ± standard deviation of independent triplicate tests
  2. * p < 0.05
  3. arxylose/rethanol was calculated from data on the xylose-only consumption phase in glucose–xylose co-fermentation (GX stage)
  4. bμ was calculated from data on the exponential growth phase of xylose fermentation (X stage)
  5. cThe specific consumption/production rates of xylose/ethanol (rxylose/rethanol) were calculated from data on X stage
  6. dThe positive operation represents overexpressed genes with lower expression in the GX stage compared to the X stage or knocked out genes with higher expression in the GX stage compared to the X stage; the negative operation represents the reverse operation