Fig. 4
Oligomeric mass profiling (OLIMP) of the different substrates after 24-h hydrolysis with the two mannanases. a Comparison of native and chemically acetylated locust bean galactomannan (LBGN and LBGA). b Comparison of native and chemically acetylated konjac glucomannan (KGMN, KGMA). c Comparison of mannanase action on native acetylated konjac glucomannan (KGMN). d Comparison of mannanase action on native acetylated spruce galactoglucomannan (SpGGM). Enzymatic hydrolysis was performed using 100 nM CjMan5A or CjMan26A. The error bars show the standard deviations of duplicate measurements. H refers to the number of hexoses in the mannooligosaccharides (Man, Glc or Gal), and Ac refers to the number of acetylations