Fig. 10

Relative expression of the PvMYB4 gene in the shoot and root of T0 stable transgenic switchgrass under the control of each of three green tissue-specific promoters measured by real-time RT-PCR. RNA was extracted from at least 10 transgenic lines of each construct at the E5 growth stage with 5 representative lines of each construct being shown. Relative quantification was performed using the standard curve method with PvUbi1 as the internal control gene. Bars represent the mean values of three independent replicates ± standard errors (vertical bars). N.T., non-transgenic switchgrass