Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789

Rebecchi, Stefano; Pinelli, Davide; Zanaroli, Giulio; Fava, Fabio; Frascari, Dario

doi:10.1186/s13068-018-1138-4

Biotechnology for Biofuels and Bioproducts

Table 1 Overview of the BD production tests: experimental conditions, oxygen mass transfer coefficient, oxygen transfer rates, liquid phase concentrations

From: Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789

Test ID	Type of medium	Test type	N (rpm)	Q_G (L/min)	k_La (1/h)^a	OTR (mmol/L/h)^b	\(q_{{{\text{O}}_{2} }}\) (mmol/g_CDW/h)^c	RQ (–)^d	CO_{2, L} (mg/L)^e	BD (g/L)^f	AC (g/L)^f	GLY (g/L)^f	Ethanol (g/L)^f	CDW (g/L)^g
SM-1	Standard	Preliminary test	Variable	0.31	Variable	Variable	Variable		0.008	8.3	11.7	0.0	0.0	3.6–6.3
SM-2	Standard	CCCD	250	0.10	6.5 ± 2.2	1.6 ± 0.5	0.7–0.4	7.0	< 0.008	17.6	0.0	9.4	0.1	2.3–3.7
SM-3	Standard	CCCD	198	0.31	8.7 ± 0.8	1.7 ± 0.2	0.7–0.5	4.0	< 0.008	15.6	0.0	9.5	1.0	2.4–3.2
SM-4	Standard	CCCD	250	0.51	13.0 ± 2.4	3.3 ± 0.5	1.2–0.8	1.8	< 0.008	17.0	0.1	7.6	0.8	2.7–4.2
SM-5	Standard	CCCD	375	0.05	13.6 ± 1.5	1.0 ± 0.2	0.4–0.2	5.7	< 0.008	19.7	0.2	6.8	1.2	2.6–4.6
SM-6	Standard	CCCD	375	0.31	21.8 ± 2.4	1.6 ± 0.2	0.6–0.3	14.1	< 0.008	18.0	0.1	5.8	0.4	2.8–5.2
SM-7	Standard	CCCD	375	0.59	32.2 ± 3.3	7.3 ± 0.7	2.3–1.4	2.1	< 0.008	17.1	0.8	3.9	0.7	3.2–5.2
SM-8	Standard	CCCD	500	0.10	46.6 ± 3.9	10.9 ± 0.8	2.6–1.7	2.5	< 0.008	18.4	1.7	0.3	0.0	4.2–6.4
SM-9	Standard	CCCD	500	0.51	80.8 ± 8.5	17.4 ± 1.8	3.6–2.6	1.9	< 0.008	16.3	2.4	0.1	0.0	4.8–6.8
SM-10	Standard	CCCD	552	0.31	84.7 ± 5.8	22.7 ± 1.2	8.4–5.2	1.1	3.1	2.0	11.0	0.0	0.0	2.7–4.3
SM-V1	Standard	Validation test	460	0.10	34.4 ± 2.9	9.4 ± 0.7	4.3–1.6	2.1	< 0.008	19.7	2.1	1.2	0.0	2.2–5.7
SM-V2	Standard	Validation test	430	0.24	26.1 ± 4.8	3.5 ± 1.1	1.5–0.6	3.0	< 0.008	16.8	1.4	0.4	0.0	2.4–5.7
SM-V3	Standard	Validation test	375	0.14	20.3 ± 0.3	1.2 ± 0.1	0.5–0.2	7.6	< 0.008	17.5	0.0	5.5	0.7	2.7–5.1
SM-V4	Standard	Validation test	405	0.51	39.4 ± 2.3	14.1 ± 0.9	5.0–2.4	1.9	< 0.008	19.3	3.8	0.0	0.0	2.8–5.8
SM-V5	Standard	Validation test	425	0.43	38.5 ± 2.5	15.1 ± 1.1	7.9–2.7	1.8	< 0.008	19.0	2.7	0.0	0.0	1.9–5.5
SM-V6	Standard	Validation test	478	0.51	72.0 ± 7.2	20.4 ± 1.2	9.8–4.1	1.8	< 0.008	7.3	10.5	0	0	2.1–5.0
SM-V7	Standard	Validation test	800	0.81	142 ± 15	28.7 ± 1.1	6.1–4.5	^h	5.2	3.2	9.9	0.0	0.0	4.7–6.4
BP-1	Glucose + CMBMⁱ	Byproduct test	405	0.50	22.0 ± 0.1	6.1 ± 0.7	1.4–0.7	^h	< 0.008	16.9	5.2	0.9	0	4.3–8.6
BP-2	Molasses + CMBMⁱ	Byproduct test	405	0.50	20.9 ± 0.2	5.8 ± 0.7	1.2–0.8	^h	< 0.008	11.5	2	0.5	0	4.9–6.9
BP-3	Molasses + CMBMⁱ	Byproduct test	460	0.10	29.5 ± 0.5	8.2 ± 1.0	1.7–1.2	^h	< 0.008	9.4	0.9	0	0	4.9–6.6
BP-4	Molasses + CMBMⁱ	Byproduct test	500	0.10	47.5 ± 3.6	13.1 ± 1.6	3.1–1.6	^h	< 0.008	10.1	3.7	0	0	4.2–8.2

^aEvaluated experimentally through the dynamic method. ^b Volumetric OTR, evaluated according to Eq. (5). ^c O₂ specific utilization rate (or biomass-specific OTR) evaluated at the onset and at the end of the actual phase of BD production, respectively. ^d Respiratory quotient, evaluated as molar ratio of carbon dioxide production rate to O₂ uptake rate. ^e Average O₂ concentration in the liquid phase during the BD production phase. ^f Liquid phase concentrations at the end of the BD production phase. ^g Cell dry weight concentration evaluated at the onset and at the end of the actual phase of BD production, respectively. ^h Not available. ⁱ Chicken meat and bone meal

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