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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Identification of two integration sites in favor of transgene expression in Trichoderma reesei

Fig. 2

FACS screening and quantification analysis of the expression levels of RFP and lipase. a, b Representative histographs of flow cytometry analysis of protoplasts from negative control strain Tu6 (a) and strains bearing the expression cassette of lipA-2A-DsRed (b). Shifted peaks towards right in a histogram represent the increase of fluorescence intensities. c Representative image showing that RFP was successfully expressed in the released protoplasts from mycelia. d Scatter plots of the fluorescent intensity and lipase activity of the 46 selected recombinant strains. Single protoplasts were sorted into 24 well microtitre plates containing MM media with 0.1% glucose (w/v), 0.1% glycerol (w/v) and 2% lactose (w/v) as carbon source and cultured for 96 h before the indicated measurement. Each data point represented one well of a 24-well plate. Correlation coefficient (r) and the P value of the correlation were provided

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