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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Identification of two integration sites in favor of transgene expression in Trichoderma reesei

Fig. 4

Characterization of the screened strain R5. a Schematic diagram of the homologous recombination occurred in strain R5. Pcbh1 represents cbh1 promoter; Tcbh1 indicates the cbh1 terminator; sp is the abbreviation of signal peptide; His indicates 6xHis tag; 2A indicates 2A peptide; DL-F, Lip-R, cbh1-F and cbh1-R were indicated primers. b Genotyping of T. reesei strain R5. Lane1, DNA Marker; Lane2, The result of diagnostic PCR using R5 genomic DNA as the template, primer cbh1-F and cbh1-R as the primer pairs; Lane3, The result of diagnostic PCR using R5 genomic DNA as the template, and primer DL-F and Lip-R as the primer pairs; Lane4, The result of diagnostic PCR using Tu6 genomic DNA as the template, primer cbh1-F and cbh1-R as the primer pairs. c, d Lipase activities and SDS-PAGE analysis of the strain Tu6, N10 and R5 from 120 h cultures containing MM media with 1% Avicel (w/v) and 1% glycerol (w/v) as the carbon source and 5 mM uridine was added. All the values in the figure are the mean value of three replicates. Error bars are the SD between these replicates

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