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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Identification of two integration sites in favor of transgene expression in Trichoderma reesei

Fig. 6

Effects of the R3 and R11 loci on the expression levels of cbh1 gene. a SDS-PAGE analysis of the supernatants of the indicated strains cultured in 144 h of MM media containing 1% Avicel (w/v) as the carbon source. b Measurement of the total cellobiohydrolase activities of the indicated strains as the same condition as in a. c Measurement of the total cellobiohydrolase activities of the indicated strains cultured in 120 h of MM media containing 2% lactose (w/v) as the carbon source and normalized the activity by biomass. d qRT-PCR analysis of the expression levels of cbh1gene. RNA was extracted from the mycelia of 96 h culture in MM media with 1% Avicel (w/v) as carbon source. All the values in the figure are the mean value of three replicates. Error bars are the SD between these replicates. Asterisks indicate significant differences (*P < 0.05; **P < 0.01; ***P < 0.001). ns not significant

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