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Table 4 Comparison of growth and cellulase activity of recombinant Y. lipolytica strains grown on 25 g/L glucose in shake flasks under normal growth condition for 2 days

From: Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose

  YLpW CYLpW CYLpO CYLpL YLxW CYLx CYLxR
Minimal media
 μmax (h−1) 0.16 ± 0.00 0.15 ± 0.01 0.15 ± 0.01 0.14 ± 0.01 0.11 ± 0.01 0.09 ± 0.00 0.08 ± 0.01
 Yx/s (g DCW/g) 0.55 ± 0.01 0.51 ± 0.01 0.54 ± 0.01 0.49 ± 0.01 0.32 ± 0.01 0.26 ± 0.00 0.24 ± 0.01
 Cellulase activity (FPU/mL) n.d. 0.18 ± 0.02 0.18 ± 0.01 0.16 ± 0.01 n.d. 0.15 ± 0.02 0.13 ± 0.01
YTD
 μmax (h−1) 0.43 ± 0.01 0.38 ± 0.01 0.37 ± 0.01 0.36 ± 0.01 0.16 ± 0.02 0.14 ± 0.01 0.12 ± 0.01
 Yx/s (g DCW/g) 0.92 ± 0.06 0.83 ± 0.04 0.82 ± 0.05 0.80 ± 0.03 0.72 ± 0.03 0.65 ± 0.02 0.63 ± 0.03
 Cellulase activity (FPU) n.d. 0.84 ± 0.05 0.86 ± 0.03 0.82 ± 0.04 n.d. 0.70 ± 0.02 0.64 ± 0.03
  1. n.d. not detectable
  2. The results were calculated from at least three biological replicates and are given as the mean values ± standard deviation
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Biotechnology for Biofuels

ISSN: 1754-6834

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