Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose

Table 4 Comparison of growth and cellulase activity of recombinant Y. lipolytica strains grown on 25 g/L glucose in shake flasks under normal growth condition for 2 days

	YLpW	CYLpW	CYLpO	CYLpL	YLxW	CYLx	CYLxR
Minimal media
μ_max (h⁻¹)	0.16 ± 0.00	0.15 ± 0.01	0.15 ± 0.01	0.14 ± 0.01	0.11 ± 0.01	0.09 ± 0.00	0.08 ± 0.01
Yx/s (g DCW/g)	0.55 ± 0.01	0.51 ± 0.01	0.54 ± 0.01	0.49 ± 0.01	0.32 ± 0.01	0.26 ± 0.00	0.24 ± 0.01
Cellulase activity (FPU/mL)	n.d.	0.18 ± 0.02	0.18 ± 0.01	0.16 ± 0.01	n.d.	0.15 ± 0.02	0.13 ± 0.01
YTD
μ_max (h⁻¹)	0.43 ± 0.01	0.38 ± 0.01	0.37 ± 0.01	0.36 ± 0.01	0.16 ± 0.02	0.14 ± 0.01	0.12 ± 0.01
Yx/s (g DCW/g)	0.92 ± 0.06	0.83 ± 0.04	0.82 ± 0.05	0.80 ± 0.03	0.72 ± 0.03	0.65 ± 0.02	0.63 ± 0.03
Cellulase activity (FPU)	n.d.	0.84 ± 0.05	0.86 ± 0.03	0.82 ± 0.04	n.d.	0.70 ± 0.02	0.64 ± 0.03

n.d. not detectable
The results were calculated from at least three biological replicates and are given as the mean values ± standard deviation

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