Fig. 2

Metabolic pathway for 1-octanol production in S. cerevisiae from glucose via fatty acid biosynthesis. A mutant version of S. cerevisiae FAS (Fas1^R1834K/Fas2) produces octanoyl-CoA which is hydrolyzed by endogenous thioesterases (TE) to the free octanoic acid [53]. A heterologous carboxylic acid reductase (CAR) from M. marinum then converts the free fatty acid to octanal which is further reduced to 1-octanol by endogenous alcohol dehydrogenases (ADH) and aldehyde reductases (ALR) [29]. CAR must be activated by the phosphopantetheinyl tranferase Sfp from Bacillus subtilis [31]. Heterologous or mutated enzymes are marked in blue. FAS^RK means FAS^R1834K