Table 1 Enzymatic properties and kinetic values of purified XYL10C, XYL10C-ΔN, XylE, XynE2, and their mutants with beechwood xylan as the substrate
Enzymes | Optimal pH | Optimal temperature (°C) | Specific activity (U/mg) | Km (mg/mL) | kcat/Km (mL/s/mg) |
|---|---|---|---|---|---|
XYL10C | 4.5 | 85 | 3200 ± 131 | 0.54 ± 0.02 | 4900 ± 201 |
XYL10C-ΔN | 4.0 | 80 | 8700 ± 403 | 0.71 ± 0.02 | 8800 ± 403 |
XYN10C-ΔN-E175Q | 5.0 | 85 | 3600 ± 167 | 0.73 ± 0.02 | 4400 ± 198 |
XylE | 5.0 | 70 | 620 ± 28 | 1.01 ± 0.03 | 490 ± 20 |
XylE-Q116E | 5.5 | 70 | 2300 ± 108 | 0.81 ± 0.02 | 1200 ± 38 |
XynE2 | 8.0 | 65 | 870 ± 37 | 0.93 ± 0.03 | 1600 ± 39 |
XynE2-Q85E | 7.0 | 65 | 1100 ± 49 | 0.72 ± 0.02 | 2200 ± 91 |