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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Biomimetic strategy for constructing Clostridium thermocellum cellulosomal operons in Bacillus subtilis

Fig. 4

Specific and quantitative enzyme assays for biomimetic cellulosomes. To quantify the enzyme activities of cellulosomal enzymes, both supernatant and intracellular materials were derived from the Type I, Type II, and Control strains and were used for enzyme assay. a Total glucanase activity assay using MUC as substrate. b Relative endoglucanase activity using dye-CMC as substrate. c Relative exoglucanase activity using PASC as substrate. d Relative xylanase activity using xylan as substrate. Results are expresed as Mean ± SD (n = 3). P ≤ 0.05, P ≤ 0.01

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