Table 1 E. coli and Y. lipolytica strains used in this study
From: Optimization of odd chain fatty acid production by Yarrowia lipolytica
Name | Relevant genotype/plasmid description | Source of references |
|---|---|---|
E. coli strains | ||
 DH5α | \( \varPhi \)80lacZ∆m15 ∆(lacZYA-argF) U169 recA1 endA1 hsdR17 (r −k , m +k ) phoA supE44 thi-1 gyrA96 relA1 λ− | Promega |
 JME740 | DH5α pKS-PUT phd1 | [32] |
 JME547 | DH5α pUB4-CRE1 | [53] |
 JME1000 | DH5α pKS-PLT tgl4 | [37] |
 JME1077 | DH5α pGEMT easy-PUT mfe1 | [36] |
 JME1128 | DH5α JMP62-pTEF-GPD1-URA3ex | [36] |
 JME1822 | DH5α JMP62-pTEF-DGA2-LEU2ex | [38] |
Y. lipolytica strains | ||
 Po1d (JMY195) | MATa ura3-302 leu2-270 xpr2-322 | [30] |
 JMY2900 | Po1d Ura+ Leu+ | [31] |
 JMY1203 | Po1d phd1::URA3ex | [32] |
 JMY3279 | Po1d ∆phd1 | This study |
 JMY3348 | Po1d ∆phd1 mfe1::URA3ex | This study |
 JMY3350 | Po1d phd1::URA3ex + LEU2 | This study |
 JMY3396 | Po1d ∆phd1 mfe1::URA3ex tgl4::LEU2ex | This study |
 JMY3433 | Po1d ∆phd1 ∆mfe1 ∆tgl4 | This study |
 JMY3576 | Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex | This study |
 JMY3776 | Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex + pTEF-GPD1-URA3ex | This study |