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Table 1 E. coli and Y. lipolytica strains used in this study

From: Optimization of odd chain fatty acid production by Yarrowia lipolytica

Name Relevant genotype/plasmid description Source of references
E. coli strains
 DH5α \( \varPhi \)80lacZ∆m15 ∆(lacZYA-argF) U169 recA1 endA1 hsdR17 (r k , m k + ) phoA supE44 thi-1 gyrA96 relA1 λ Promega
 JME740 DH5α pKS-PUT phd1 [32]
 JME547 DH5α pUB4-CRE1 [53]
 JME1000 DH5α pKS-PLT tgl4 [37]
 JME1077 DH5α pGEMT easy-PUT mfe1 [36]
 JME1128 DH5α JMP62-pTEF-GPD1-URA3ex [36]
 JME1822 DH5α JMP62-pTEF-DGA2-LEU2ex [38]
Y. lipolytica strains
 Po1d (JMY195) MATa ura3-302 leu2-270 xpr2-322 [30]
 JMY2900 Po1d Ura+ Leu+ [31]
 JMY1203 Po1d phd1::URA3ex [32]
 JMY3279 Po1d ∆phd1 This study
 JMY3348 Po1d ∆phd1 mfe1::URA3ex This study
 JMY3350 Po1d phd1::URA3ex + LEU2 This study
 JMY3396 Po1d ∆phd1 mfe1::URA3ex tgl4::LEU2ex This study
 JMY3433 Po1d ∆phd1 ∆mfe1 ∆tgl4 This study
 JMY3576 Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex This study
 JMY3776 Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex + pTEF-GPD1-URA3ex This study