Fig. 4

CtPMO1 insoluble reaction products hydrolyzed by endo-1,4-beta-glucanase followed by Br₂ oxidation with PASC as substrate. Insoluble reaction products (residual PASC) upon incubation of 0.5% PASC with CtPMO1 in 10 mM HAc-NH₄Ac (pH 5.0) and 1 mM ascorbate at 50 °C for 48 h. CtPMO1 insoluble reaction products hydrolyzed by endo-1,4-beta-glucanase using TLC analysis (a) and MALDI-TOF–MS (b). CtPMO1 insoluble reaction products hydrolyzed by endo-1,4-beta-glucanase followed by Br₂ oxidation for 30 min (c) and for 60 min (d) using MALDI-TOF–MS analysis. C1-oxidized oligosaccharides (m/z + 16), C4- or C6-oxidized oligosaccharides (m/z − 2), lactones (m/z -4) of C1- (m/z + 16) and C4- or C6-oxidized oligosaccharides (m/z − 2), C4- and C1-oxidized oligosaccharides (m/z + 14), C6- and C1-oxidized oligosaccharides (m/z + 30), and C6-, C1- and C4-oxidized oligosaccharides (m/z + 28). M, standard cellulo-oligosaccharides (G1–G7). CK, samples upon incubation of insoluble reaction products (residual PASC) in 10 mM HAc-NH₄Ac (pH 5.0) at 50 °C for 10 min with inactive endo-1,4-beta-glucanase treated at 100 °C for 30 min