Fig. 4
Effect of catalase on cellulose hydrolysis in the presence and absence of MnAOx and reduced oxygen. Pretreated wheat straw was incubated at 50°C, pH 5 for 96 h under conditions of ambient air (approximately 20% O2) or reduced oxygen (approximately 0.3% O2). The enzyme cocktail consisted of CTec3 (8.4 mg protein/g cellulose) and 0.22 mg/g cellulose catalase (blue diamonds), CTec3 + 0.22 mg/g cellulose MnAOx + catalase (red triangles), CTec3 + MnAOx (green circles), or CTec3 alone (black squares). a, b Show the fractional conversion of cellulose to glucose, while c, d Show the concentrations of gluconic acid. The curves shown in a, b are model fits