Revealing the transcriptomic complexity of switchgrass by PacBio long-read sequencing

Table 1 A summary of the initial and processed Iso-Seq data from six pooled tissue types

Library	# SMRT cell	#ROI	#FL	#Non-FL	#Reads < 300 bp	#Artificial concatemers	Mean size of FL (bp)	% FL	#Consensus sequences
Gel
PB0938	2	57,613	17,273	33,863	6455	22	2019	30	5337
PB0939	2	60,270	10,318	41,604	8325	23	2911	17	2393
PB0940	2	35,226	485	2371	32,345	25	406	10	208
PB0941	2	60,887	17,463	11,806	31,566	52	524	29	5984
PB0942	2	103,211	31,359	48,990	22,817	45	1115	30	8779
SageELF
PB0988	2	264,829	90,710	156,094	16,832	1193	2079	34	34,066
PB0989	2	313,987	91,557	205,554	15,603	1273	2640	29	30,392
PB0990	2	330,259	105,964	205,387	17,295	1613	2462	32	35,342
PB0991	2	266,928	89,911	154,177	21,440	1400	2012	34	29,121
PB0992	2	270,609	99,371	140,062	29,964	1212	1541	37	29,266
PB0993	2	290,900	109,275	138,236	41,563	1826	1118	38	30,534
PB0994	2	276,923	105,743	116,597	51,857	2726	823	38	27,245
PB0995	2	67,946	37,849	19,610	10,281	206	537	56	14,243
PB0996	2	273,102	15,683	249,333	6013	2073	6407	6	3169
PB0997	2	369,770	36,156	319,644	10,540	3430	4262	10	9694
Summary
Total: 15	30	3042,460	859,117 (28.2%)	1843,328 (60.6%)	322,896 (10.6%)	17,119 (0.6%)	N/A	N/A	265,773

ISSN: 2731-3654