Fig. 4

Affinity of the purified CmChi1 for polysaccharides. The reaction mixture of 2-mL contained 150 μg of CmChi1 and 1 g/L of one of the polymeric substrates in 50 mM sodium citrate buffer couple with 0.5 M NaCl (pH 5.2). The affinity mixture was incubated at 4 °C with rotary shaking at 1000 rpm. Samples from different time intervals were immediately centrifuged, and the unabsorbed protein in the supernatant was measured. The adsorbed protein was calculated as the amount of protein in the control minus that in the supernatant. All experiments were performed in triplicate