Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Table 2 Editing efficiencies of rfp via different CRISPR-Cas systems and repair mechanisms

CRISPR-Cas system	DSB repair	Red colonies	Colorless colonies	Total^a	Efficiency (%)
CRISPR-Cas9	Native NHEJ	48	0	48	0
	Heterologous NHEJ	48	0	48	0
	HR^b	0	23	23	100
CRISPR-Cpf1	Native NHEJ	48	0	48	0
	Heterologous NHEJ	48	0	48	0
	HR	23	0	23	0

^aColonies were picked randomly and red fluorescence was measured on a microplate reader
^bColonies were picked randomly and identified by PCR. One of the PCR products was verified by sequencing

ISSN: 2731-3654