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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthin

Fig. 4

Hydrolysis of Ast-E by Lipase-YH. a TLC analysis. Lane 1: free astaxanthin. Lane 2: reaction at 0 h. Lane 3: Lipase-YH produced by X33-YH. Lane 4: pro-ALIP produced by mα-proalip-X33-1. Lane 5: mALIP produced by 9K-malip-GS115. Lane 6: mALIP produced by zα-malip-X33-1. b HPLC analysis of Ast-E hydrolysis catalyzed by Lipase-YH enzyme powder dissolved in water. c HPLC spectrum at 0 h. d HPLC spectrum at 2 h. e MS of peak at RT = 16.006 min. Lipase-YH hydrolyzed Ast-E into free astaxanthin, as confirmed by TLC and HPLC. When Lipase-YH enzyme powder dissolved in water was used as catalyst, free astaxanthin yield reached 57.1%

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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