Fig. 3

Growth, substrate utilization and wax ester production of aerobic ADP1-g cultivations with 20 mM acetate and 15 mM butyrate in 50 ml JM medium. a The growth (closed circles), determined as optical density at 600 nm wavelength, and organic acid utilization (acetate, open stars; butyrate, open triangles) by ADP1-g is presented. The data points are averaged values and error bars indicate standard deviation from triplicate experimental repeats. In some cases, the error bars are smaller than the symbol. b Thin layer chromatography analysis of wax ester synthesized by ADP1-g from acetate and butyrate. The time point samples analyzed with TLC correspond to the same presented in (a). In above figures a and b, ADP1-g cells in JM medium (including 0.3 g/l of yeast extract and 25 µg/l of chloramphenicol diluted in ethanol) represents the growth (black and white square) and wax ester profiles, respectively, of ADP1-g cells when cultivated without acetate and butyrate supplementation