Fig. 3

a PCA score plot and loading plot derived from metabolic profiling of BUOHSE and DC7. The score plot showed distinct separation of DC7 and BUOHSE, represented by blue and yellow circles, respectively. The loading plot revealed good correlation of acetyl-CoA and (iso-) citrate with the DC7 data set. b Relative intensity of intracellular acetyl-CoA and (iso-) citrate levels in BUOHSE and DC7. Asterisks indicate significant differences in the strains (*p ≤ 0.05; **p ≤ 0.01). c Intracellular concentration of acetyl-CoA, butanoyl-CoA and free CoA in BUOHSE and DC7. The error bars indicate standard deviation obtained from three replicates