Fig. 2

Effect of enzyme sources of DHCR24 on 7-DHC production. a Phylogenetic analysis of DHCR24 protein sequences selected in this study. b 7-DHC production in strains with DHCR24s from diversity species. Those DHCR24s that could not realize 7-DHC accumulation are denoted by red triangle. c Western-blotting of lysates from cells expressing polyhistidine-tag-attached DHCR24s from the selected sources. Cells were cultured in YPD medium and harvested at 40 h (ethanol consumption phase). Extracts were probed with anti-polyhistidine and anti-GAPDH (as loading control). The relative expression level of each DHCR24 is displayed as the gray scale of anti-polyhistidine band divided by that of anti-GAPDH. The error bars represent standard deviation calculated from triplicate experiments. Hs, Homo sapiens; Mm, Mus musculus; Dr, Danio rerio; Ec, Equus caballus; Gg, Gallus gallus; Xt, Xenopus tropicalis; Bt, Bos Taurus; At, Arabidopsis thaliana; Gh, Gossypium hirsutum; Cg, Cryptococcus gattii; Tg, Trypanosoma grayi