Fig. 4
From: Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction
Effect of ΔERG6 on 7-DHC production. a GC/TOF-MS analysis of the fermentation products of strains SyBE_Sc0125XJ03 (red) and SyBE_Sc0125XJ04 (blue). I, squalene; II, 7-DHC; III, zymosterol; IV, lanosterol. The relative accumulations of squalene (b) zymosterol (c), and lanosterol (d) in strains SyBE_Sc0125XJ03 (control) and SyBE_Sc0125XJ04 (ΔERG6) were determined using those in the control strain for normalization. e Improvement of 7-DHC accumulation via blocking the competitive path (ΔERG6) and supplementing another copy of DHCR24 in locus ERG6. The error bars represent standard deviation calculated from triplicate experiments