Fig. 6

Neighbor-joining phylogenetic tree (a) based on partial 16S rRNA genes of Cloacimonetes _bOTUs identified in this study and the comparison of the protein content for three newly reconstructed Cloacimonetes genomes (b, c). Bootstrap support values higher than 50% were displayed next to the nodes on the tree (a). Heat map representation of the relative _bOTU abundance was calculated for each sample representing the time series of the year monitoring of the 20 AD reactors analysed in this study, and was organised by the cluster affiliation (separated by dashed vertical lines). Bold dashed vertical line separates the agricultural and bio-waste treating units from the WWTP-ADs. An asterix highlights the OTUs from the previous Goux et al. study [27]. Circular representation (b) of the Candidatus Cloacamonas acidaminovorans (strain Evry) protein content (circle one from the outside; hypothetical proteins are highlighted in red colour, block size corresponds to the protein size in aa), and its similarity to the respective homologous proteins in the metagenome-reconstructed genomes of three other Cloacimonetes species (Cloa 1, 2 and 3 corresponding, respectively, to the _bOTUs-492, 5 and 12; three internal circles, respectively). Prophage regions identified in the genome of the strain Evry are highlighted with blue lines. Average protein similarity (for the homologous proteins) for Cloa 1, 2 and 3 to the Candidatus Cloacamonas acidaminovorans (strain Evry) proteins is shown on the graph c. The boxes represent the interquartile similarity range and the error bars show the 95% confidence intervals