A year of monitoring 20 mesophilic full-scale bioreactors reveals the existence of stable but different core microbiomes in bio-waste and wastewater anaerobic digestion systems

Table 2 Primers pairs used in this study to specifically amplify 16S rRNA genes from bacteria and archaea

Domain	Primer name	Sequence 5′→3′	Variable region	Average amplicon size (bp)	In silico specificity^a	Ref.
Bacteria	S-D-Bact-0909-a-S-18	ACTCAAAKGAATWGACGG	V6–V8	484	0–78.8% 1–89.6%	[60]
Bacteria	S-*-Univ-1392-a-A-15	ACGGGCGGTGTGTRC	V6–V8	484	0–78.8% 1–89.6%	[60]
Archaea	S-D-Arch-0519-a-S-15	CAGCMGCCGCGGTAA	V4–V6	526	0–79.3% 1–93.7%	[60]
Archaea	S-D-Arch-1041-a-A-18	GGCCATGCACCWCCTCTC	V4–V6	526	0–79.3% 1–93.7%	[60]

^aThe in silico specificity against the rRNA gene sequences deposited in SILVA database v132; the first number refers to the number of mismatches, while the percentage refers to the proportion of taxa that was targeted specifically

ISSN: 2731-3654