Fig. 1
Effect of the concentrations of reductant and O2 on saccharification of Avicel with Cellic® CTec2. a, b Reactions under air saturated conditions (21% v/v O2) using various concentrations of ascorbic acid (AscA). c, d Reactions with 5 mM AscA using various oxygen saturation levels in the headspace. The left panels (a, c) show production of Glc4gemGlc and the right panels (b, d) show glucan conversion. Reaction mixtures contained 10% (w/w) DM of Avicel and 4 mg/g DM of Cellic® CTec2, in 50 mM sodium acetate buffer at pH 5.0 and were incubated at 50 °C. The error bars represent standard deviations for three independent experiments