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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Fig. 6

Effect of various constant H2O2 feeding rates on the saccharification of steam-exploded birch with Cellic® CTec2. a The production of Glc4gemGlc and b the glucan conversion. All reactions were carried out with 10% (w/w) DM of birch, 2 mg/g DM of Cellic® CTec2 in the absence of AscA, in 50 mM sodium acetate buffer at pH 5.0 and 50 °C with constant supply of H2O2 (600 µL h−1) using H2O2 stock solutions with appropriate concentrations (Table 1). To ensure anaerobic conditions, all reactions were carried out with constant sparging of nitrogen at a flow rate of 100 mL min−1, with the exception of the aerobic control reaction (O2), which was sparged with air at the same flow rate. “N2” and “N2 Cys” stand for the anaerobic control reactions; the latter was carried out in the presence of 0.025% (w/v) l-cysteine hydrochloride monohydrate. All control reactions were run without addition of H2O2. Since the glucan fraction of this substrate was only 43.9%, here, in contrast to all other reactions reported in this study, Cellic® CTec2 was dosed at 2 mg (rather than 4 mg) protein/g DM. Error bars for glucan conversion represent standard deviations of two technical replicates

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