The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Table 2 Saccharification yields and total supply of H₂O₂ in 48 h hydrolysis experiments carried out in bioreactors with continuous feeding of H₂O₂

Substrate	Experiment	H₂O₂ feed rate (µM h⁻¹)	Glucose release at T_48h (g L⁻¹)	Glucan conversion at T_48h (% of theoretical)	Cumulative H₂O₂ (mM)	Cumulative H₂O₂ (µmol g⁻¹ DM)
Avicel	O₂	–	64.0	62.5	–	–
	O ^a₂	–	48.1	47.0	–	–
	N₂	–	52.6	51.3	–	–
	90	90	69.2	67.6	4.32	43.2
	300	300	60.8	59.3	14.4	144
	600	600	50.6	49.4	28.8	288
	Decrease	Variable^b	71.1	69.4	5.4	54
	Addition	Variable^b	67.3	65.7	7.2	72
Norway spruce	O₂	–	70.6	72.0	–	–
	N₂	–	52.9	54.5	–	–
	90	90	72.7	75.6	4.32	43.2
	300	300	77.4	80.7	14.4	144
	600	600	62.8	65.3	28.8	288
SEB	O₂	–	35.6	72.9	–	–
	N₂	–	38.3	78.4	–	–
	N ^Cys₂	–	35.5	72.7	–	–
	90	90	43.4	89.0	4.32	43.2
	300	300	40.8	83.6	14.4	144
	600	600	35.1	72.0	28.8	288

Experimental details are provided in Fig. 4 (Avicel), 5 (Norway Spruce) and 6 (SEB). All reactions were all performed at 10% DM content
^aAerobic control reaction carried out in the absence of reducing agent (AscA)
^bDetails of the H₂O₂ feeding regime are provided in the caption to Fig. 4

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