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Table 2 Saccharification yields and total supply of H2O2 in 48 h hydrolysis experiments carried out in bioreactors with continuous feeding of H2O2

From: The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Substrate Experiment H2O2 feed rate (µM h−1) Glucose release at T48h (g L−1) Glucan conversion at T48h (% of theoretical) Cumulative H2O2 (mM) Cumulative H2O2 (µmol g−1 DM)
Avicel O2 64.0 62.5
O a2 48.1 47.0
N2 52.6 51.3
90 90 69.2 67.6 4.32 43.2
300 300 60.8 59.3 14.4 144
600 600 50.6 49.4 28.8 288
Decrease Variableb 71.1 69.4 5.4 54
Addition Variableb 67.3 65.7 7.2 72
Norway spruce O2 70.6 72.0
N2 52.9 54.5
90 90 72.7 75.6 4.32 43.2
300 300 77.4 80.7 14.4 144
600 600 62.8 65.3 28.8 288
SEB O2 35.6 72.9
N2 38.3 78.4
N Cys2 35.5 72.7
90 90 43.4 89.0 4.32 43.2
300 300 40.8 83.6 14.4 144
600 600 35.1 72.0 28.8 288
  1. Experimental details are provided in Fig. 4 (Avicel), 5 (Norway Spruce) and 6 (SEB). All reactions were all performed at 10% DM content
  2. aAerobic control reaction carried out in the absence of reducing agent (AscA)
  3. bDetails of the H2O2 feeding regime are provided in the caption to Fig. 4
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