Substrate
|
Experiment
|
H2O2 feed rate (µM h−1)
|
Glucose release at T48h (g L−1)
|
Glucan conversion at T48h (% of theoretical)
|
Cumulative H2O2 (mM)
|
Cumulative H2O2 (µmol g−1 DM)
|
---|
Avicel
|
O2
|
–
|
64.0
|
62.5
|
–
|
–
|
O
a2
|
–
|
48.1
|
47.0
|
–
|
–
|
N2
|
–
|
52.6
|
51.3
|
–
|
–
|
90
|
90
|
69.2
|
67.6
|
4.32
|
43.2
|
300
|
300
|
60.8
|
59.3
|
14.4
|
144
|
600
|
600
|
50.6
|
49.4
|
28.8
|
288
|
Decrease
|
Variableb
|
71.1
|
69.4
|
5.4
|
54
|
Addition
|
Variableb
|
67.3
|
65.7
|
7.2
|
72
|
Norway spruce
|
O2
|
–
|
70.6
|
72.0
|
–
|
–
|
N2
|
–
|
52.9
|
54.5
|
–
|
–
|
90
|
90
|
72.7
|
75.6
|
4.32
|
43.2
|
300
|
300
|
77.4
|
80.7
|
14.4
|
144
|
600
|
600
|
62.8
|
65.3
|
28.8
|
288
|
SEB
|
O2
|
–
|
35.6
|
72.9
|
–
|
–
|
N2
|
–
|
38.3
|
78.4
|
–
|
–
|
N
Cys2
|
–
|
35.5
|
72.7
|
–
|
–
|
90
|
90
|
43.4
|
89.0
|
4.32
|
43.2
|
300
|
300
|
40.8
|
83.6
|
14.4
|
144
|
600
|
600
|
35.1
|
72.0
|
28.8
|
288
|
- Experimental details are provided in Fig. 4 (Avicel), 5 (Norway Spruce) and 6 (SEB). All reactions were all performed at 10% DM content
- aAerobic control reaction carried out in the absence of reducing agent (AscA)
- bDetails of the H2O2 feeding regime are provided in the caption to Fig. 4