Fig. 1From: Optimization of biomass pretreatments using fractional factorial experimental designReducing sugars released from substrates before and after 12 h enzymatic hydrolysis and their percentages of acetyl bromide soluble lignin, crystalline cellulose and silica: a elephant grass pretreated with acid–alkali (EA) and b with acid–organosolv (EO). Reducing sugars (mg/g substrate) are indicated by grey bars in the left axis, while lignin (black squares), cellulose (grey squares) and silica (white circles) are indicated in the right axis. Error bars are standard deviation values from replicatesBack to article page