Fig. 1

XacAbf51 is a thermotolerant Abf and enhances saccharification of delignified sugarcane bagasse. Circular dichroism spectrum of XacAbf51 (a) and thermal denaturation profile of the enzyme assessed by CD (b) and DSC (c). Residual activity of XacAbf51 over pNP-Araf after incubation at 50 °C for up to 72 h (d). Sugar released from delignified sugarcane bagasse by Celluclast (238 µg) (e) or T. reesei RUT-C30 enzyme cocktail (238 µg) (f) in the absence or presence of XacAbf51 (13 µg). **P value ≤ 0.01; ***P value ≤ 0.001 (one-tailed Student’s t test)