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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization

Fig. 5

Comparison of commercial fungal cellulase with SKLMY complex on 0.25% (w/v) softwood pulp as substrate. The soluble fraction of reducing sugar ends was quantified after 2 days of incubation at optimal reaction conditions (60 °C for cellulosomal native and synthetic complexes at pH 5.8, and 50 °C at pH 5.0 for fungal enzyme preparation, respectively). The SKLMY complex was mixed with varying amounts (% of all cohesin-binding positions on CipA8) of native components (SM901 mutant extract) or an equimolar ratio of recombinant enzymes (n = 47, Additional file 4 for enzymatic composition). Enzyme loadings were as follows: Cellic CTec2, 7.6 µg per reaction; synthetic cellulosome complexes (each 1 µg); non-complexed enzyme control (− CipA: 13.2 µg); cellulosome complexes contain 3 µg of β-glucosidase as additive in the reaction mixture. Substrate loading was 1.25 mg per reaction. Bars represent average values ± standard deviation from three independent enzyme reactions

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