Fig. 5

Correlation of TaFAR1-fused GFP intensity and fatty alcohol production in T. reesei. Flow cytometry-sorted spores were cultivated and subjected to fatty alcohol quantification (a) and hyphae fluorescence detection (b). Strains were grouped according to the fluorescence value/OD600 (b). 10 or 1 strain from each group was randomly selected for fatty alcohol quantification (a, b) or Tafar1 expression detection (c), respectively. Results of hexadecanol yield are the mean of experiments on ten separate strains and error bars indicate standard deviations (n = 10 ± SD). Asterisks indicated a significant difference (p < 0.05) according to Student’s t test. Con: pyr4-TU-6 strain, G6: Ppdc-gfp-TU-6 strain, T1, T2, T3: Ppdc-Tafar1-gfp-TU-6 strains randomly selected from groups with different fluorescence value/OD600 (200–300, 300–400, 400–450), respectively