Table 1 Typical parameters for purification of β-glucosidases from Penicillium citrinum UFV1
Purification step | Total activity (mU) | Total protein (mg) | Specific activity (U/mg of protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Crude extract | 3464.1 | 34.2 | 101.4 | 1.0 | 100.0 |
Ammonium sulfate precipitation | 2666.0 | 12.9 | 205.9 | 2.0 | 77.0 |
Phenyl sepharose | |||||
PcβGlu1 | 341.6 | 0.9 | 398.3 | 3.9 | 9.9 |
PcβGlu2 | 547.2 | 1.6 | 338.1 | 3.3 | 15.8 |
Superdex 200 | |||||
PcβGlu2 | 111.1 | 0.3 | 349.2 | 3.4 | 3.2 |