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Table 1 Typical parameters for purification of β-glucosidases from Penicillium citrinum UFV1

From: Penicillium citrinum UFV1 β-glucosidases: purification, characterization, and application for biomass saccharification

Purification step Total activity (mU) Total protein (mg) Specific activity (U/mg of protein) Purification (fold) Yield (%)
Crude extract 3464.1 34.2 101.4 1.0 100.0
Ammonium sulfate precipitation 2666.0 12.9 205.9 2.0 77.0
Phenyl sepharose
 PcβGlu1 341.6 0.9 398.3 3.9 9.9
 PcβGlu2 547.2 1.6 338.1 3.3 15.8
Superdex 200
 PcβGlu2 111.1 0.3 349.2 3.4 3.2