Table 2 Thermoinactivation constant (kd) and half-life (T1/2) for PcβGlu1 in the presence of ammonium sulfate and for PcβGlu2 in the presence of BSA when incubated at different temperatures
Incubation temperature | ||||
|---|---|---|---|---|
50 °C | 60 °C | 70 °C | 80 °C | |
kd PcβGlu1 | – | – | 1.4 × 10−6 ± 5 × 10−7 | 4.4 × 10−3 ± 8 × 10−4 |
kd PcβGlu2 | 1.4 × 10−5 ± 2 × 10−6 | 7.3 × 10−4 ± 3 × 10−5 | – | – |
T1/2 PcβGlu1 | – | – | 9000 ± 900 | 2.6 ± 0.5 |
T1/2 PcβGlu2 | 800 ± 100 | 16 ± 1 | – | – |
kd (s−1) | T1/2 (min) | |||