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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolases

Fig. 2

Hydrolytic products of different polysaccharides analysed by thin-layer chromatography (TLC). Polysaccharides were hydrolysed by a Gal53A or b Xyn10B. Samples were incubated overnight (16 h) at pH 6.5 and 60 °C with 5.0 mg/L enzyme and 0.5% substrate in 0.1 M MOPS reaction buffer. Arabinan (A), galactan potato (G(P)), galactan lupin (G(L)), pectic galactan potato (PG(P)), soluble wheat arabinoxylan (AX(s)), insoluble wheat arabinoxylan (AX(i)), barley β-glucan (BBG), lichenin (L), glucuronoxylan (GX), xylan (birch) (X(B)), xylan (oat spelt) (X(H)), negative control (−), hydrolysate (+), standards: xylose, arabinose, glucose, galactose (1), xylose, xylobiose, xylotriose, xylotetraose, xylopentatose (2), glucose, cellobiose, cellotriose, cellotetraose, cellopentose (3), galactose (4)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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