Skip to main content
Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolases

Fig. 4

Hydrolysis pattern of investigated arabinoxylanolytic enzymes on purified arabinoxylo- and xylooligosaccharides. Arrows indicate the first cleavage site of the enzymes. Arrows with asterisk indicate potential cleavage sites. Due to the design of the experiment, it was not possible to identify the actual cleavage site. The hydrolysis experiments were performed overnight at 60 °C and pH 6.5 with 2 mg/L enzyme and 20 mg/L pure oligosaccharide or oligosaccharide mixture. Analysis of hydrolysis products was performed using HPAEC-PAD. Xylanases (Xyn), arabinofuranosidases (Arf), arabinoxylan arabinofuranohydrolase (Axh), xylosidases (Xyl)

Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us