Fig. 4

Alkane production from acetate. The selected alkane-producing A. baylyi strain was cultivated in a bioreactor in minimal salts medium in fed-batch mode. The feed contained 80 g/L acetic acid, and the feed rate was manually controlled to keep the acetate concentration in the reactor between 30 and 60 mM. Acetate consumption was calculated based on the measured acetate concentrations in the reactor and the volume of the acetic acid solution fed to the reactor