l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain

Table 2 Relative quantity (RQ) of GAL2 gene expression levels for cultivations of S. cerevisiae DS61180 under microaerobic conditions in shake flasks on the substrates d-glucose, d-galactose, and l-arabinose in minimal medium

Substrates	RQ of GAL2 (%)
Single substrates
d-Glucose (negative control)	0.52 (± 0.04)
d-Galactose (positive control and reference)	100.01 (± 3.71)
l-Arabinose	21.29 (± 0.84)
Mixed substrates
d-Glucose/l-arabinose mixture (growth on d-glucose in presence of l-arabinose)	0.23 (± 0.05)
d-Glucose/l-arabinose mixture (growth on l-arabinose after d-glucose depletion)	4.06 (± 0.22)

Substrates were either provided as single substrates or as mixture of d-glucose and l-arabinose. SGA1 was used as endogenous control and the cultivation on d-galactose served as reference. Given errors indicate standard deviations from three measurements of biological duplicates (n = 6)

ISSN: 2731-3654