Fig. 5

Expression of lignocellulolytic enzymes by WT or mutant plasmids in fermentor. Cells containing WT or mutant plasmid were grown in a 5 L fermentor for 72 h. Curves of enzymatic activity and wet cell weight were plotted for cells expressing Est1E (a), RuCelA (c), Xyn-CDBFV (e) or MAN330 (g). SDS-PAGE of the supernatant at different time points is shown in (b, d, f, h)