Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana

Table 1 Phenotypic traits

Line	Height (cm)	Mass (mg)	% CWR
tra2 comt-1
WT	46.8 ± 5.3	43.1 ± 9.9	75.4 ± 1.7
tra2	46.5 ± 4.7	37.5 ± 11.3	75.2 ± 1.1
comt-1	48.3 ± 4.6	44.4 ± 11.3	74.5 ± 2.1
tra2 comt-1	48.7 ± 3.8	42.6 ± 10.8	74.0 ± 2.2
c4h-3 comt-4
WT	48.2 ± 4.9	53.5 ± 15.0	74.9 ± 1.8
c4h-3	50.5 ± 3.3	55.8 ± 12.9	76.0 ± 2.5
comt-4	50.5 ± 5.6	54.9 ± 9.5	75.7 ± 2.3
c4h-3 comt-4	50.2 ± 4.1	54.8 ± 12.3	74.2 ± 1.4
4cl1-1 comt-4
WT	51.3 ± 4.0	43.7 ± 7.7	77.8 ± 1.2
4cl1-1	50.2 ± 3.8	43.6 ± 7.5	77.0 ± 1.7
comt-4	52.5 ± 3.6	43.9 ± 7.1	76.9 ± 1.4
4cl1-1 comt-4	51.6 ± 3.0	43.9 ± 6.6	77.1 ± 1.4

Height and mass were determined on fully senesced primary inflorescence stems of the three double mutants, wild type (WT), and the corresponding parental lines. The data represent the average ± standard deviation of at least 24 replicates per line. The cell-wall residue (CWR) was determined gravimetrically after extraction and is expressed in % dry weight ± standard deviation (n = 10). No significant differences were observed at the 0.01 significance level (ANOVA, pairwise comparison with Holm correction)
WT wild type

ISSN: 2731-3654