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Table 2 Lignin amount and composition

From: Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana

Line AcBr S/G % H % G % S % 5H
tra2 comt-1
 WT 11.2 ± 0.9a 0.63 ± 0.08a 0.4 ± 0.1a 60.9 ± 2.9a 38.5 ± 2.9a 0.24 ± 0.05a
 tra2 10.0 ± 1.4ab* 0.79 ± 0.11b 0.6 ± 0.2a 55.4 ± 3.3b 43.7 ± 3.3a 0.27 ± 0.05a
 comt-1 10.9 ± 0.7ab 0.02 ± 0.01c 0.5 ± 0.2a 96.9 ± 1.4c 1.5 ± 1.4b 0.99 ± 0.37b
 tra2 comt-1 9.6 ± 1.0b 0.01 ± 0.00c 0.7 ± 0.2a 96.8 ± 0.4c 1.4 ± 0.5b 0.99 ± 0.29b
c4h-3 comt-4
 WT 11.3 ± 0.8a 0.52 ± 0.03a 0.7 ± 0.1a 65.2 ± 1.2a 33.9 ± 1.2a 0.16 ± 0.02a
 c4h-3 8.1 ± 0.8b 1.05 ± 0.07b 1.2 ± 0.4b.c 48.2 ± 1.6b 50.5 ± 1.9b 0.18 ± 0.01b
 comt-4 11.6 ± 1.9a 0.06 ± 0.01c 1.0 ± 0.3a.b 92.2 ± 1.0c 5.6 ± 0.8c 1.25 ± 0.14c
 c4h-3 comt-4 7.9 ± 0.3b 0.13 ± 0.01d 1.5 ± 0.2c 85.4 ± 0.8d 11.5 ± 0.8d 1.54 ± 0.39c
4cl1-1 comt-4
 WT 11.8 ± 0.7a 0.62 ± 0.18a 1.2 ± 0.2a 61.7 ± 6.9a 36.9 ± 6.8a 0.16 ± 0.04a
 4cl1-1 8.3 ± 1.4b 0.90 ± 0.04b 2.0 ± 0.3b, c 51.7 ± 1.2b 46.2 ± 1.2b 0.16 ± 0.02a
 comt-4 10.2 ± 1.0c 0.11 ± 0.02c 1.6 ± 0.5a, c 87.2 ± 2.3c 9.8 ± 1.9c 1.49 ± 0.55b
 4cl1-1 comt-4 6.9 ± 0.5d 0.18 ± 0.02d 2.4 ± 0.6b 82.0 ± 1.6d 14.5 ± 1.4d 1.08 ± 0.39b
  1. Lignin amount and composition of the three double mutants, wild type (WT), and the corresponding parental lines (n = 10) (± SD). The lignin amount was determined via the AcBr method and is expressed as % CWR. The lignin composition was determined via thioacidolysis. The relative amounts of the different lignin units were calculated based on the total thioacidolysis yield. The S/G ratio was calculated based on the absolute values for S and G (expressed in μmol mg−1 AcBr lignin). Significance groups are indicated with the same letter in superscript and different letters represent significant differences at the 0.01 significance level (ANOVA or Kruskal–Wallis, pairwise comparison with Holm correction). *p value 0.051 after pairwise comparison with Holm correction
  2. WT wild type