Genetic repertoires of anaerobic microbiomes driving generation of biogas

Table 1 Overview of the samples from agricultural biogas plants and laboratory reactors used in this study, showing the different operational parameters and the type of sequencing approaches applied

Fermenter	Operational parameters		Database assembly sequencing approach	Database evaluation sequencing approach
Fermenter	Substrate	Temperature, °C	DNA seq. HiSeq and MinIon sequencing	DNA seq. HiSeq sequencing	RNA seq. HiSeq sequencing
Agricultural biogas plant
1	Maize silage, cattle slurry, horse + pig + cattle manure, organic waste	41	×
2	Maize silage, pig + cattle slurry, chicken dung, organic waste	54	×		×
3	Maize silage, grass silage	52	×		×
4	Maize silage, grass silage	40	×
5	Maize silage, cattle slurry, horse manure	51		×
6	Maize silage	40		×
7	Grass silage, maize silage, grain, cattle slurry	40		×	×
Laboratory reactors
R1/R2 d0	Maize silage	41		×	×
R1 7 d	Maize silage	41		×	×
R1 21 d	Maize silage	41		×	×
R1 42 d	Maize silage	41		×	×
R1 84 d	Maize silage	41		×	×
R2 7d	Maize silage	35		×	×
R2 21d	Maize silage	41		×	×
R2 42 d	Maize silage	41		×	×
R2 84 d	Maize silage	41		×	×

ISSN: 2731-3654