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Table 1 Overview of the samples from agricultural biogas plants and laboratory reactors used in this study, showing the different operational parameters and the type of sequencing approaches applied

From: Genetic repertoires of anaerobic microbiomes driving generation of biogas

Fermenter Operational parameters Database assembly sequencing approach Database evaluation sequencing approach
Substrate Temperature, °C DNA seq. HiSeq and MinIon sequencing DNA seq. HiSeq sequencing RNA seq. HiSeq sequencing
Agricultural biogas plant
 1 Maize silage, cattle slurry, horse + pig + cattle manure, organic waste 41 ×   
 2 Maize silage, pig + cattle slurry, chicken dung, organic waste 54 ×   ×
 3 Maize silage, grass silage 52 ×   ×
 4 Maize silage, grass silage 40 ×   
 5 Maize silage, cattle slurry, horse manure 51   ×  
 6 Maize silage 40   ×  
 7 Grass silage, maize silage, grain, cattle slurry 40   × ×
Laboratory reactors
 R1/R2 d0 Maize silage 41   × ×
 R1 7 d Maize silage 41   × ×
 R1 21 d Maize silage 41   × ×
 R1 42 d Maize silage 41   × ×
 R1 84 d Maize silage 41   × ×
 R2 7d Maize silage 35   × ×
 R2 21d Maize silage 41   × ×
 R2 42 d Maize silage 41   × ×
 R2 84 d Maize silage 41   × ×