Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for lignocellulosic biofuel production

Table 1 Comparisons of xylose fermentation performance of the XUSE strain with those of previously reported xylose-fermenting strains

Strain	Genetic background	Fermentation condition		Ethanol yield (g/g)
XUSE (this study)	xylA3, TAL1, XKS1, Δgre3, Δpho13,* evolved	Microaerobic batch in serum bottle, synthetic medium (20 g/L xylose)	OD 0.2	0.43
			OD 2	0.45
			OD 10	0.4
SXA-R2P-E	xylA3, tal1, XKS1, Δgre3, Δpho13*, evolved	Microaerobic batch in serum bottle, synthetic medium (20 g/L xylose)	OD 0.2	0.4
			OD 2	0.4
			OD 10	0.4
		Anaerobic batch in bioreactor, synthetic medium (40 g/L xylose)		0.45 [15]
RWB217	xylA, TAL1, TKL1, RPE1, RKI1, Δgre3	Anaerobic batch in fermenter, synthetic medium (20 g/L xylose)		0.43 [14]
H131-A3-AL^CS	xylA, xyl3, TAL1, TKL1, RPE1, RKI1, evolved	Anaerobic batch in bioreactor, synthetic medium (40 g/L xylose)		0.41 [13]
TMB 3424	xyl1, xyl2, XKS1, TKL1, RPE1, RKI1, ∆gre3, evolved	Anaerobic batch in bioreactor, synthetic medium (60 g/L xylose)		0.36 [12]

ISSN: 2731-3654