Fig. 5
From: Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
Pentalenene production in S. cerevisiae. a The S. cerevisiae host engineered for the production of antimalarial precursor amorphadiene was re-engineered to produce pentalenene by overexpressing pentalenene synthase (PentS) in a plasmid. ERG10 acetyl-CoA acetyltransferase, IDI1 isoprenyl diphosphate isomerase, ERG20 farnesyl disphosphate synthase. Genes in blue arrows (upc2-1, tHMGR, ERG20 and PentS) are overexpressed. b Production titer and c cell growth (OD600) with CSM medium and YEP medium supplemented with 1.8% galactose/0.2% glucose