Fig. 7From: The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilusTpRfx1 binding to the promoter regions of cellulase genes. The indicated amounts of purified TpRfx1190-367 were mixed with about 150 ng of FAM-labeled EMSA probes. EMSA probes lacking the FAM label and the promoter region of β-tubulin gene were used for competitive EMSA and as a negative control, respectively. BSA alone and TRX-His-S from E. coli cells containing the empty vector pET-32a(+) were used as controlsBack to article page