De novo transcriptome assembly of the bamboo snout beetle Cyrtotrachelus buqueti reveals ability to degrade lignocellulose of bamboo feedstock

Luo, Chaobing; Li, Yuanqiu; Liao, Hong; Yang, Yaojun

doi:10.1186/s13068-018-1291-9

Biotechnology for Biofuels and Bioproducts

Table 5 Cellulase activities of C. buqueti in different tissues

From: De novo transcriptome assembly of the bamboo snout beetle Cyrtotrachelus buqueti reveals ability to degrade lignocellulose of bamboo feedstock

	Tissues	Female	Male	Larvae	Substrates
Endoglucanase	Mouthpart	167.574 ± 56.143cA	36.944 ± 7.116bB	22.227 ± 2.293aB	Carboxy methyl cellulose (CMC)
	Foregut	584.753 ± 91.215aA	168.065 ± 27.839aB	0aB
	Midgut	547.303 ± 55.846aA	198.834 ± 35.686aB	228.197 ± 49.354aB
	Hindgut	119.618 ± 46.375bcA	74.403 ± 10.015aA	0aB
	Total intestine	333.488 ± 56.600abcA	150.491 ± 25.473aA	0aA
	Mouthpart + total intestine	237.788 ± 49.687bcA	122.701 ± 41.611aA	17.936 ± 4.6191aB
Exoglucanase	Mouthpart	0aA	54.314 ± 12.635aA	30.288 ± 6.118aA	Microcrystalline celluose (MC)
	Foregut	0aB	15.939 ± 3.643bA	44.996 ± 4.774aA
	Midgut	5.021 ± 1.112aA	76.401 ± 17.143aA	84.693 ± 12.384aA
	Hindgut	27.639 ± 9.401aA	214.597 ± 54.711bA	0aB
	Total intestine	0aA	37.614 ± 4.551bA	0aA
	Mouthpart + total intestine	6.768 ± 2.796aA	93.996 ± 19.370bA	2.821 ± 1.155aA
β-glucosidase	Mouthpart	34.231 ± 10.168bB	185.758 ± 53.732bA	7.575 ± 2.613aB	Salicin
	Foregut	29.294 ± 11.822bB	239.177 ± 100.275bA	0.4454 ± 0.107aB
	Midgut	235.814 ± 59.925aB	1744.827 ± 50.604aA	6.487 ± 1.058aB
	Hindgut	22.959 ± 7.533bA	0bA	0.4217 ± 0.111aA
	Total intestine	53.002 ± 17.693bA	382.107 ± 124.953bA	2.429 ± 0.669aA
	Mouthpart + total intestine	36.031 ± 10.066bA	326.273 ± 117.143bA	5.788 ± 0.447aA
Laccase	Mouthpart	0.049 ± 0.001bB	0.168 ± 0.003aA	0.053 ± 0.003bB	2,2,-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
	Foregut	0.004 ± 0.001bA	0.192 ± 0.042aA	0.029 ± 0.001bA
	Midgut	0.111 ± 0.011bB	0.372 ± 0.061aB	5.101 ± 0.532aA
	Hindgut	0.065 ± 0.001bA	0.113 ± 0.014aA	0.003 ± 0.001bA
	Total intestine	0.738 ± 0.231aA	0.376 ± 0.072aA	0.699 ± 0.099bA
	Mouthpart + total intestine	0.121 ± 0.038bB	0.256 ± 0.008aA	0.061 ± 0.004bB
Manganese peroxidase	Mouthpart	0.327 ± 0.068aA	0.917 ± 0.228aA	0.326 ± 0.037bA	2,6-Dimethyl phenol (2,6-DMP)
	Foregut	0.322 ± 0.025aA	0.235 ± 0.006aA	0.057 ± 0.003bA
	Midgut	0.124 ± 0.027aB	0.475 ± 0.069aB	2.162 ± 0.453aA
	Hindgut	0.558 ± 0.1129aB	1.372 ± 0.191aA	0.068 ± 0.002abA
	Total intestine	0.152 ± 0.010aA	0.737±0.171aA	0.893 ± 0.195bA
	Mouthpart + total intestine	0.082 ± 0.016aC	0.324 ± 0.027aB	0.772 ± 0.023abA
Lignin peroxidase	Mouthpart	0.146 ± 0.022bA	0.459 ± 0.130bA	0.013 ± 0.003bcB	Veratryl alcohol (VA)
	Foregut	0.013 ± 0.001bA	0cA	0.002 ± 0.001bcA
	Midgut	0.437 ± 0.061bA	0.471 ± 0.034bcA	0.043 ± 0.012aB
	Hindgut	0.051 ± 0.010abB	1.453 ± 0.289aA	0.008 ± 0.002cB
	Total intestine	0bA	0.065 ± 0.014bcA	0.026 ± 0.008bA
	Mouthpart + total intestine	0bB	0.067 ± 0.016bcA	0.012 ± 0.003bcA

A total of 165 female imagos, 165 male imagos and 165 larvae were sampled to determine enzyme activity. Five biological replicates were conducted for each treatment. Descriptive data are expressed as mean ± SEM. Different lowercase letters in the same column indicate significant differences in the different intestinal sections at the 0.05 level (n = 5); Different capital letters in the same line indicate significant differences at different developmental stage at the 0.05 level (n = 5)

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ISSN: 2731-3654

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