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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Expression of an endoglucanase–cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi

Fig. 6

Digestion of Avicel with purified L. starkeyi enzymes. All enzymes used in this assay were expressed and purified from L. starkeyi and loaded at equimolar concentrations, TeTrCBHI at 16.72, TrEGII at 13.28, and Fusion 3 at 31.24 mg/g cellulose. In addition, A. niger β-glucosidase was added at 1.0 mg/g cellulose. The assay enzymes were loaded on 5 mg Avicel/mL at 40 °C and pH 5.0 in 20 mM acetate buffer. Conversion percentages were calculated based on HPLC determinations of released monomeric glucose

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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