The physiological effect of heavy metals and volatile fatty acids on Methanococcus maripaludis S2

Table 1 Analysis of acetate (Ac) and propionate (Pr) concentrations at the end of each experiment

Sample	−c (mmol_Ac L⁻¹)	Ac (mmol_Ac L⁻¹)	−c (mmol_Pr L⁻¹)	Pr (mmol_Pr L⁻¹)
1Ac	9.81	9.19 ± 0.04	na	na
2Ac	48.34	46.47 ± 2.48	na	na
3Ac	85.74	82.43 ± 1.84	na	na
1Pr0Ac	0.34	1.39 ± 0.27	9.96	9.82 ± 0.49
1Pr1Ac	9.81	9.18	9.96	8.43
2Pr1Ac	8.92	10.39 ± 1.00	41.04	38.71 ± 0.67
3Pr1Ac	10.69	9.43 ± 0.57	75.48	72.51 ± 2.02
1Cu2Ac	47.63	41.40 ± 1.02	na	na
2Cu2Ac	44.47	41.60 ± 1.58	na	na
3Cu2Ac	43.85	41.98 ± 0.65	na	na
1Cu3Ac	84.77	77.72 ± 1.27	na	na
2Cu3Ac	84.16	76.08 ± 1.94	na	na
3Cu3Ac	85.37	77.38 ± 2.29	na	na

Batch cultivations of M. maripaludis were performed at 37 °C, 140 rpm, 2.9 bar. The values 1, 2, 3 refer to the increasing concentrations of each compounds applied individually and simultaneously. (1,2,3) Ac: 12.2, 60.9, 121.9 mmol L⁻¹; (1,2,3) Pr: 10.4, 52.0, 104.1 mmol L⁻¹ 12.2, 60.9, 121.9 mmol L⁻¹; Cu (1.9, 4.4, 6.3 µmol L⁻¹). The negative control (−c) refers to medium that did not receive inoculum of cells and therefore is used as a reference value
na not applied

ISSN: 2731-3654